Genetic characteristics of coxsackievirus A6 in hospitalized patients with hand, foot and mouth disease in autumn and winter in Qingdao 2019
10.3760/cma.j.cn112866-20210129-00020
- VernacularTitle:2019年秋冬季青岛地区住院手足口病患者感染柯萨奇病毒A组6型基因特征分析
- Author:
Chao ZHAO
1
;
Jing JIA
;
Ruifang WANG
;
Xia WANG
;
Yiqiu WAN
;
Jun HAN
;
Fachun JIANG
;
Chen GAO
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，北京 102206
- Keywords:
Hand;
foot and mouth disease;
Enterovirus;
Coxsackievirus A6;
Gene characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(3):257-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the prevalence of coxsackievirus A6 (CV-A6) in hospitalized patients with hand, foot and mouth disease in autumn and winter of 2019, and the genetic characteristics of VP1 region and the relation-ship between germline evolution.Methods:A total of 104 throat swabs from hospitalized patients with hand, foot and mouth disease in Qingdao in autumn and winter of 2019, and screen positive specimens were collected using enterovirus universal real-time PCR technology. Then the universal typing primers in the VP1 region were used to amplify the positive samples, the amplified segments were sequenced and BLAST comparison was performed. CV-A6 VP1 full-length primers were used to amplify the BLAST comparison of CV-A6 positive samples, sequence the ampli-fied products, analyze the homology of nucleotides and amino acids through DNAstar and MEGA software, and construct a phylogenetic tree.Results:Among 104 throat swab samples, 60 were positive for enterovirus, and the overall positive rate was 57.7%, of which the positive rate of CV-A6 was 26.9% (28/104), and the rate of coxsackievirus A16 (CV-A6) was 30.8% (32/104). Twenty-eight CV-A6 positive samples were subjected to full-length amplification, sequencing, comparison and homology analysis of the VP1 region, and 22 positive sequences were obtained. The nucleotide homology was 93.6% to 99.9%, and the amino acid homology was 98.0%-100%. Phylogenetic analysis showed that 22 CV-A6 strains belonged to the D3 subtype in the D genotype.Conclusions:The pathogens causing hospitalized hand, foot and mouth disease in Qingdao in autumn and winter of 2019 were CV-A6 and CV-A16. The prevalent strains of CV-A6 were mainly D3 subtypes.
