Protective effect of CBD and nimodipine against rat cortical neuronal injury in vitro

VernacularTitle:CBD和尼莫地平对培养大鼠皮层神经元损伤的保护作用
Author: Ke-Wan WANG ¹ ; Xin-Min BAO ; Si-Yun SHU ; Chang-Cai CHEN
Author Information

1. Nanfang Hospital
Keywords: traumatic brain injury; glutamate; neurotrophic factor; neuron Cultured
From: Journal of Southern Medical University 2000;20(6):523-526
CountryChina
Language:Chinese
Abstract: Objective To investigate the protective effects of CBD and Nimodipine against injuries secondary to glutamate-inuced or traumatic injury in rat cortical neurons in vitro. Methods Mix-cultured cortical neurons of SD rat were subjected to either glutamate injury or mechanical damage. The degree of injury were detected by cell count of trypan blue staining and measurement of lactate dehydrogenase (LDH) activity in the culture medium. Results When combined treatment with CBD and Nimodipine was used separately, moderate protective effects against the injury of cultured cortical neurons induced by glutamate or trauma were observed, which was obvious when compared with the injured group without treatment (P<0.01). When combined treatment with CBD and Nimodipine was administered, strong protective effect were observed than that with treatment using CBD or Nimodipine alone (P<0.01). Conclusion It is suggested that CBD combined with Nimodipine are synergetic. Combined use of different excitotoxic antagonists can be fruitful in the therapeutic intervention of secondary traumatic damage.