Effect of xanthoxylin on A23187-and calcium-induced platelet aggregation and cytosolic free-Ca2+ level

VernacularTitle:花椒油素对A23187和钙调节血小板及细胞内游离钙作用的研究
Author: Yun-Hai JIN ¹ ; Yi-Ping JINAG ; Ji-Hong LIN
Author Information

1. Department of Pharmacology
Keywords: xanthoxylin; calcium ion; calcimycin; platelet aggregation; cytosolic free Ca2+; fluorescence analysis
From: Journal of Southern Medical University 2000;20(6):513-514
CountryChina
Language:Chinese
Abstract: Objective To study the effects of xanthoxylin on platelet aggregation and freeCa2+ level induced by CaCl2 and A23187. Methods Platelet aggregation and free Ca2+ level in the platelet cytosol were measured by turbidimetry and fluorimetry of Fura-2/AM. Results Xanthoxylin at the concentration of 0.037, 0.185, 0.924, 9.240, 92.40 μmol/L significantly inhibited CaCl2 and A23187-induced platelet aggregation, with the inhibition rates ranging from 9.0% to 67.1% for CaCl2 and from 18.1% to 72.5% for A23187. A23187-induced free-Ca2+ level in the platelet cytosol was also significantly reduced (from 12.8% to 63.4%). Conclusion The inhibition rate of platelet aggregation was positively correlated with the reduction rate of cytosolic free-Ca2+ level by xanthoxylin.