Effects of three kinds of stable expressive antisense c-myc RNA on the growth and proliferation of vascular smooth muscle cell
10.3321/j.issn:1673-4254.2000.06.005
- VernacularTitle:三种反义c-myc RNA稳定表达对血管平滑肌细胞生长、增殖的影响
- Author:
Rong ZENG
1
;
Xiao-Rong LIU
;
Jin LI
;
Yun DAI
;
Jing AN
Author Information
1. First Military Medical University
- Keywords:
antisense nucleic acid;
oncogene;
proliferation;
vascular smooth muscle cell;
gene therapy
- From:
Journal of Southern Medical University
2000;20(6):499-503
- CountryChina
- Language:Chinese
-
Abstract:
Ojective In order to understand the effects of continuous and stable expression of antisense genes on the growth and proliferation of the smooth muscle cells. Method Three combinant retrovial expression vectors, aM1, aM2 and aM3, which was respectively loaded with reverse fragment of c-myc exon 1, 2 and 3, were transferred into rat arterious smooth muscle cells and assayed 1 month after their stable expression. Result aM1 and aM2 could inhibit Myc expression (respectively reduced to 59.7%, 81.3%), and the expression of proliferat cellular nuclear antigen (PCNA) (respectively reduced to 52.4%, 51.9%). aM1 and aM2, especially the latter also inhibited cellular proliferative activity, aM2 slightly hindered cellular cycle and DNA synthesis too, while the effects of aM3 turned out to be contrary to that of aM1 and aM2. In addition, compared with transient expression, the effects of introduced genes were variable in target cells, and the changes brought about by the transfection process would reduce and disappear in a short time with the lowering of cellular compensation. Conclusions Blocking different regions of c-myc can achieve different effects and the blokade of the transcription activity regions usually produces enhanced growth inhibition effects. Moreover, target cells may have some 'correction mechanism ' against the introduced foreign genes.
