Etiology of human adenoviruses associated with intestinal infections in children in Beijing
10.3760/cma.j.cn112866-20200916-00245
- VernacularTitle:北京市儿童肠道感染相关腺病毒的病原学研究
- Author:
Liying LIU
1
;
Yuan QIAN
;
Liping JIA
;
Huijin DONG
;
Runan ZHU
Author Information
1. 首都儿科研究所病毒研究室，儿童病毒病病原学北京市重点实验室，北京，100020
- Keywords:
Children;
Diarrhea;
Human adenovirus;
Fiber
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(2):182-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the prevalence and genetic characteristics of human adenovirus (HAdV) in healthy children and diarrheal children in Beijing.Methods:A total of 1 834 fecal samples were collected from diarrheal children younger than 6 years old in outpatients from January, 2015 to December, 2017 and from healthy children with the same age group from May, 2016 to February, 2017. The hexon and fiber genes of HAdV were detected for genotyping and the sequences analysis, respectively.Results:The detection rate of HAdVs in healthy children (1.5%, 8/536) was significantly lower than that in children with diarrhea (7.4%, 96/1 298) ( χ2=23.62, P<0.001). HAdV-41 was the dominant type detected both in the healthy children and diarrheal outpatients. There was no gender difference in HAdV-positive children with diarrhea. Except for newborns with no positive case, there was no difference in the detection rate of HAdV among the other age groups. There was no obvious seasonality of HAdV prevalence. Phylogenetic analysis showed that HAdV-41 strains circulating in Beijing belonged to three evolutionary branches, and strains with 15-amino acid truncated in the shaft of fiber were found. Conclusions:HAdV was one of the important pathogens causing diarrhea in children in Beijing from 2015 to 2017, with HAdV-41 as the predominant type.
