Development of an immunochromatographic assay for rapid detection of human adenovirus
10.3760/cma.j.cn112866-20201225-00313
- VernacularTitle:一种快速检测人腺病毒的免疫层析分析方法的建立
- Author:
Shuhui LIU
1
;
Na LEI
;
Jingdong SONG
;
Lishu ZHENG
;
Jing GE
;
Yu LAN
;
Kun QIN
;
Jianfang ZHOU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒学和病毒病重点实验室，北京 102206
- Keywords:
Human adenovirus;
Immunochromatography assay;
Hexon;
Monoclonal antibody
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(1):101-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quick and convenient method for detecting human adenoviruses (Human adenoviruses, HAdV) based on immunochromatographic assay (ICA).Methods:Two antibody clones, 3C11 and 7E6 were found to bind to all tested HAdVs and then subsequently processed into ICA. The specificity and sensitivity were evaluated using representative strains of the respiratory HAdV types, including HAdV-1, 2, 3, 4, 5, 6, 7, 10 and a gastroenteric type HAdV-41 together with the original throat swabs of 10 HAdV patients confirmed by nuclear acid testing (NAT).Results:The ICA exhibited high specificity to HAdVs and its detection limitation ranged from 0.16 to 10 3 half tissue culture infectious dose (TCID 50)/ml for different types of HAdVs. All clinic samples with successful virus isolation tested by this ICA showed positive result . Conclusions:The ICA developed in the present study will be suitable for HAdVs screening in clinic setting, especially for those of respiratory types.
