Analysis of surveillance results for H5N6 subtype avian influenza virus from population and live poultry market environment in Changsha city, 2016-2018
10.3760/cma.j.cn112866-20200508-00156
- VernacularTitle:2016—2018年长沙市人群及活禽市场环境H5N6亚型禽流感病毒监测分析
- Author:
Rusheng ZHANG
1
;
Jingfang CHEN
;
Zheng HUANG
;
Dong YAO
;
Wen YE
;
Xiaolei LIU
;
Lingzhi LI
;
Xinhua OU
;
Biancheng SUN
Author Information
1. 长沙市疾病预防控制中心 410007
- Keywords:
Influenza-like cases;
Live poultry market;
Avian influenza virus;
H5N6 subtype;
Gene
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(1):96-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To carry out the monitoring of H5N6 subtype avian influenza virus (AIV) in the population infection and live poultry markets (LPMs) environmental pollution in Changsha city from 2016 to 2018. Laboratory data were provided to prevent human infection with H5N6 subtype AIV.Methods:A total of 6 909 throat swab specimens from the population and 1 719 environmental specimens from LPMs were collected in Changsha city from 2016 to 2018. The real-time RT-PCR method was used for the detection of influenza virus in type A, H5, H7, H9 and N6 subtypes. Eighty-two AIV nucleic acid-positive specimens were subjected to high-throughput nucleotide sequencing, and then BLAST similarity and amino acid (aa) key site analyses were performed on the sequencing results.Results:One H5N6 subtype AIV nucleic acid was detected from 6 909 population throat swab specimens, 927 (53.93%) positive specimens of A-type AIV nucleic acids and 193 (11.23%) positive specimens of both H5 and N6 subtypes were detected from 1 719 LPMs environmental specimens. Fourteen genomic sequences of H5N6 subtype AIV were obtained with high-throughput nucleotide sequencing, and aa key site analyses showed that there were 6 basic aa in 338-347 aa of viral HA protein-linked peptide, which was highly pathogenic to birds. Receptor binding site (RBS) 238-240 aa (corresponding to H3 type influenza virus 226-228 aa) is QSG or QRG, and the receptor is characterized by avian origin. The 290th resistance gene locus of NA protein did not show R290K mutation, and was sensitive to NA inhibitor (Duffy/osoterosine phosphate). The E627K and D701N(I) mutations in the viral PB2 protein indicate that the virus is pathogenic.Conclusions:Human infected with H5N6 subtype AIV in Changsha city is sporadic, and the H5N6 subtype AIV of LPMs is seriously polluted. It is necessary to further strengthen the AIV surveillance of LPMs.
