Analysis of antigenicity-related gene evolution and vaccine-matching rate of influenza A virus prevalent strain in Liaoning province in 2018-2019
10.3760/cma.j.cn112866-20190731-00120
- VernacularTitle:2018—2019年辽宁省甲型流感病毒流行株HA基因进化与疫苗株匹配度分析
- Author:
Haibo SUN
1
;
Shuang LIU
;
Yichun SONG
;
Lulu WANG
;
Baihong SUN
;
Qian ZHANG
;
Lingling MAO
;
Yingwen SUN
;
Wenqing YAO
Author Information
1. 辽宁省疾病预防控制中心，沈阳 110005
- Keywords:
Influenza A;
Antigenicity;
Genetic evolution analysis;
Vaccine efficacy.
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(1):75-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Analyze the hemagglutinin(HA) gene of influenza A strains in Liaoning Province from 2018 to 2019, calculate and evaluate the matching degree of influenza A strains and vaccine strains.Methods:49 influenza A strains from 2018 to 2019 were selected for sequencing, and the HA gene was analyzed for evolution; the mutation of antigen epitopes, glycosylation sites and receptor binding sites were studied; the matching degree between the epidemic strain and the vaccine was analyzed by pepidope model.Results:The 2018-2019 influenza pandemic season was concentrated in November-March, and the main pandemic strain was H1N1 pdm virus. Through evolutionary analysis, H1N1 pdm virus belonged to the 6B.1 branch, and the seasonal H3N2 strain was distributed in the 3C.2a branch, all of which belonged to the same branch as the vaccine strain this year. Some strains mutated at epitope and receptor binding sites. In some strains, glycosylation sites increased or were absent compared with vaccine strains, and no new glycosylation sites were present. The Pepitope model was used to evaluate the vaccine effect. H1N1 pdm virus vaccine of this year showed good protective effect, but the seasonal H3N2 vaccine strain showed negative protective effect for epidemic strain, which could not play an effective protective role. Conclusions:In 2018-2019, H1N1 epidemic strain in Liaoning Province had some variation, and the matching degree between H3N2 subtype epidemic strain and 2018-2019 vaccine strain was low. We should pay close attention to the gene variation of the epidemic strains and update the vaccine strains in time.
