Evaluation of the Cepheid Xpert Xpress Flu/RSV Assay in the detection of viral pathogens in children with acute respiratory tract infection
10.3760/cma.j.cn112866-20200922-00250
- VernacularTitle:赛沛Xpert Xpress Flu/RSV Assay在儿童急性呼吸道感染病原学检测中的应用评价
- Author:
Xiaolei GUAN
1
;
Yali DUAN
;
Wei WANG
;
Meng ZHANG
;
Xiangpeng CHEN
;
Qiuping LI
;
Junhong AI
;
Chunyan LIU
;
Yun ZHU
;
Zhengde XIE
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院 北京市儿科研究所 感染与病毒研究室 儿科重大疾病研究教育部重点实验室 儿童呼吸道感染性疾病研究北京市重点实验室 中国医学科学院儿童危重症感染诊治创新单元，北京 100045
- Keywords:
Influenza virus;
Respiratory syncytial virus;
Children;
Molecular diagnosis
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(1):62-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the performance of the Cepheid Xpert Xpress Flu/RSV assay (Xpert) in the detection of children infected with influenza virus (Flu) or respiratory syncytial virus (RSV).Methods:Nasopharyngeal specimens were collected from children who showed symptoms of respiratory infection and tested FluA, FluB and RSV by Xpert and sequencing assay respectively side by side. Discordant result were tested with a laboratory-developed real-time PCR for resolution.Results:A total of 388 nasopharyngeal swabs (NAPs) from children with acute respiratory tract infection were analyzed and the result showed 91.75-94.85% agreement between two tests. The sensitivity of FluA, FluB and RSV detected by Xpert and sequencing assay were 99.21% (125/126) vs. 92.86% (117/126), 100.00% (109/109) vs. 84.40% (92/109) and 100.00% (52/52) vs. 40.38% (21/52), respectively. The specificity of FluA, FluB and RSV detected by Xpert were all lower than that of the sequencing assay: 95.42% (250/262) vs 99.24% (260/262), 99.28% (277/279) vs 99.64% (278/279) and 99.70% (335/336) vs 100.00% (336/336). The positive predictive values (PPV) of FluA, FluB and RSV detected by Xpert were lower than those of the sequencing assay: 91.30% (126/138) vs. 98.33% (118/120), 98.18% (108/110) vs. 98.92% (92/93) and 98.11% (52/53) vs. 100.00% (21/21), respectively. The negative predictive values (NPV) of FluA, FluB and RSV detected by Xpert were higher than those of the sequencing assay: 99.60% (251/252) vs. 96.67% (261/270), 99.64% (279/280) vs. 94.27% (280/297) and 100.00% (337/337) vs. 91.60% (338/369).Conclusions:The Cepheid Xpert Xpress Flu/RSV assay is a sensitive, reliable and rapid assay for the detection of FluA, FluB and RSV in pediatrics.
