Genetic characterization of varicella-zoster virus in Liaoning province in 2019, China
10.3760/cma.j.cn112866-20201116-00288
- VernacularTitle:2019年辽宁省水痘-带状疱疹病毒基因特征分析
- Author:
Hong GUO
1
;
Shuang HAO
;
Ruiping HU
;
Yan WANG
;
Songtao XU
Author Information
1. 内蒙古医科大学基础医学院生物化学与分子生物学教研室，呼和浩特 010110
- Keywords:
Varicella-zoster virus;
Genotype;
Vaccine strain;
Wild-type strain
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(1):57-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic characteristics of varicella-zoster virus (VZV) in Liaoning province in 2019.Methods:Vesicle fluid samples were collected from 32 individuals with suspected VZV infection in Liaoning province from 2019. Nucleic acids were extracted, the suspected samples were screened by real-time fluorescent quantitative PCR. Single nucleotide polymorphisms (SNP) sites on ORF22, ORF38 and ORF62 were detected and analyzed in three open reading frames (ORFS) of positive samples. The samples and sequence data were collected and the gene sequences were compared to determine the genotype of the virus strain, and the vaccine strain was identified from the wild strain.Results:In the 32 suspected samples, VZV-positive strains were detected in 27 samples, all belonged to wild-type strain of Clade 2. Compared with Clade 2 referenced strains, the homology of nucleotide and amino acid for all 10 samples were 98.85%-100% and 96.9%-100%, respectively. There was a synonymous mutation (A→G) in position 37990 of LN-14 strain. The nucleotide homology of ORF22 showed that all 27 samples were on the same branch with the Clade 2 referenced strains and had a vaccine breakthrough cases.Conclusions:This study reveals that the VZV strains circulating in Liaoning province in 2019 were all wild-type strains belonging to Clade 2.
