Analysis of whole genome characteristics of 1 clinical subgenus C human adenovirus strain isolated from Hunan province in 2014
10.3760/cma.j.cn112866-20201210-00302
- VernacularTitle:2014年湖南省C亚属人腺病毒1株临床分离株的全基因组特征分析
- Author:
Ruoqun MA
1
;
Xingyui XIANG
;
Hong ZHANG
;
Fangcai LI
;
Chaoyan HUANG
;
Ailii CUI
;
Yan ZHANG
;
Wenbo XU
;
Naiying MAO
;
Zhen ZHU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒和病毒病重点实验室，北京 102206
- Keywords:
Hunan province;
Human mastadenovirus C;
Genetic characterization
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(1):49-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the genetic characteristics of subgenus C human adenovirus (HAdV-C) strain isolated in Hunan in 2014.Methods:An HAdV-C strain named Hunan2014-s024 was isolated from throat swab collected from a child with severe acute respiratory infection in Changsha city of Hunan province in 2014. The whole genome sequence (WGS) was obtained by segmented amplification and sequence splicing. Database of HAdV-C was constructed with the strain from this study and the HAdV-C representative strains from GenBank. The whole genome characteristics was analyzed by using bioinformatics software.Results:Strain Hunan2014-s024 has the highest homology with the HAdV-C strain (MK041244-CHN-2006) isolated from stool sample of a healthy child in Shanxi province in 2006, and the genome of strain MK041244-CHN-2006 was used as the backbone, and penton based gene was replaced by gene fragment from MK041246-chn-2012 strain isolated from a child with acute lower respiratory tract infection in Hunan province in 2012.Conclusions:Our result indicated that 2014 Hunan strain(Hunan2014-s024)is a recombinant virus of 2006 Shanxi strain(MK041244-CHN-2006)and 2012 Hunan strain(MK041246-CHN-2012). Due to the change of pathogenicity, it is necessary to strengthen the molecular epidemiological surveillance of HAdV-C to understand its potential disease burden and public health significance.
