PREPARATION OF ANTI-IDIOTYPIC ANTIBODIES SPECIFIC FOR ANTI-HEL AND ANALYSIS OF THEIR FUNCTIONAL MIMICRY
- Author:
Mingyuan LI
1
;
Yu XIAO
;
Liying XIAO
;
Hong LI
;
Zhonghua JIANG
;
Jiawan MU
;
Daoruo WANG
Author Information
1. West China University of Medical Sciences
- Keywords:
anti-idiotypic antibody;
functional mimicry;
hen egg-white lysozyme
- From:
Chinese Medical Sciences Journal
2000;15(2):124-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective. This study is to investigate the functional mimicry by using anti-idiotypic antibodies of enzymes.Results.Eight hybridomas strains secreting anti-idiotypic antibodies were selected and characterized. It was shown that two of eight anti-idiotypic antibodies secreted by two hybridomas(1A10C9 and 2A11 C1B3) could mimic HEL catalytic activity to lyse Micrococcus lysodeikticus and that the catalytic effect of mixed anti-idiotypic antibodies of 1A10C9 and 2A11C1B3 was stronger than that of one of them, but less than HEL.Conclusion. The results demonstrated that the anti-idiotypic antibodies that could mimic enzyme activity existed in the idiotype network during anti-enzymatic immune response.
