Antigenic and genetic characteristics of influenza A(H1N1)pdm09 virus during the 2018-2019 influenza surveillance year in the mainland of China
10.3760/cma.j.cn112866-20200623-00194
- VernacularTitle:2018—2019流感监测年度中国大陆A(H1N1)pdm09流感病毒的抗原和基因特性分析
- Author:
Minju TAN
1
;
Yanhui CHENG
;
Xiyan LI
;
Hejiang WEI
;
Jia LIU
;
Xiang ZHAO
;
Ning XIAO
;
Dayan WANG
;
Weijuan HUANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家流感中心卫生健康委员会医学病毒和病毒病重点实验室，北京 102206
- Keywords:
Influenza A(H1N1)pdm09 virus;
Antigenicity;
Genetic characteristic
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(6):610-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The antigenic and genetic characteristics of influenza A(H1N1)pdm09 virus isolated from the mainland of China during the 2018-2019 influenza surveillance year were analyzed.Methods:Two thousand nine hundred and fifty-eight influenza A(H1N1)pdm09 virus strains in the 2018-2019 influenza surveillance year were analyzed by hemagglutination inhibition test. The hemagglutinin(HA) gene of 279 influenza A(H1N1)pdm09 virus strains was sequenced and analyzed. The representative strains of the dominant clades were performed for antigenic characteristics using post-vaccination human antisera.Results:Two thousand eight hundred and sixty-one (97%, 2 861/2 958) viruses characterized were antigenically similar to A/Michigan/45/2015. All HA gene of the sequenced viruses belonged to 6B.1 clade, and 269(96%, 269/279) viruses belonged to 6B.1A subclade. Compared with the vaccine virus, it had the common amino acid substitutions of S74R, S164T and I295V in the HA protein. There were several small groups with common amino acid substitutions in the 6B.1A subclade, and 51% sequenced viruses had S183P amino acid substitution in this subclade. The result of antigenic analysis using post-vaccination human antiserums showed that most of the representative strains were well inhibited by the sera.Conclusions:The antigenicity of influenza A(H1N1)pdm09 viruses in the mainland of China in 2018-2019 influenza surveillance year matched well with the corresponding vaccine strain, but the HA gene had genetically diverse characteristic.
