Application of routine HIV infection testing results in recent infection testing algorism
10.3760/cma.j.cn112866-20200921-00249
- VernacularTitle:应用HIV感染的常规检测指标辅助判定新发感染的研究
- Author:
Yikui WANG
1
;
Ping LIU
;
Jing LIU
;
Jin YANG
;
Xing DUAN
;
Tao YANG
;
Song DUAN
;
Yao XIAO
;
Jibao WANG
;
Cong JIN
Author Information
1. 德宏傣族景颇族自治州疾病预防控制中心，芒市 678400
- Keywords:
HIV infection;
RITA;
LAg-EIA;
CD4 T cell count;
Viral load testing
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(6):594-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The result of virological and immunological testing in newly diagnosed HIV cases identified in Dehong prefecture of Yunnan province from 2015 to 2017 was analyzed retrospectively to examine the feasibility of using routine HIV testing result in recent infection testing algorism (RITA).Methods:HIV recently tested result and routine HIV testing result were collected from 1 152 newly diagnosed HIV cases between 2015 and 2017 in Dehong prefecture of Yunnan province, the limiting antigen avidity enzyme immunoassay (LAg-EIA) was used to determine the recent infection and long-term infection. And the distribution of CD4 + T cell count, viral load, the S/CO value of antibody enzyme-linked immunological reaction (ELISA) and p31 bands appeared in Western blotting assay (WB) were analyzed in the recently infected HIV cases and long-term infected cases. Further, different RITAs integrating routine HIV testing result were compared in their capacity to determine the recently infected cases. Results:Among 1 152 newly diagnosed HIV cases, 205 recently infected cases and 947 long-term infected cases were determined by LAg-EIA. The proportion of CD4 + T cell count<200/μl, the proportion of ELISA sample/cutoff ratio (S/CO) value > 20, and the proportion of appearing p31 band in WB in recently infected HIV cases were significantly lower than those in long-term infected HIV cases. The RITAs that integrating LAg-EIA with the reclassification criteria of CD4 + T cell count <200 cells/μl or viral load value <1 000 copies (CPs)/ml reclassified 30 cases and 31 cases as long-term infection, respectively. The RITA that integrating LAg-EIA with both the reclassification criteria of CD4 + T cell count <200 cells/μl and viral load value <1 000 CPs/ml reclassified 60 cases as long-term infection. Conclusions:Routine HIV testing result can be integrated in RITA, and RITA can improve the accuracy of identifying recent HIV infection.
