PROTECTIVE EFFECT OF MELATONIN ON NEURAL CELLS AGAINST THE CYTOTOXICITY OF OXYRADICALS
- Author:
Yihua AN
1
;
Enzhong LIU
;
Xiangzhen LIU
;
Fuming YANG
;
Fengping HAN
;
Qinshun DAI
Author Information
1. the First Affiliated Hospital of Harbin Medical University
- Keywords:
melatonin;
apoptosis;
amyloid β-protein;
cytotoxicity
- From:
Chinese Medical Sciences Journal
2000;15(1):40-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective. To investigate the exact mechanism of melatonin to prohibit the apoptosis of neural cells induced by various kinds of cytotoxic agents.Methods. We used the methods of phase contrast microscopy, MTT assay and hoechst dye staining to check this mechanism in SKNSH and U251 cell lines.Results. Both 2mmol/L H2O2 and 0.5 μ mol/L amyloid β- protein (Aβ) induce these two cell lines die via apoptosis. Either melatonin or glutathione can significantly protect both cell lines. The protective effect of 10 μ mol/L melatonin is as same as that of 60 μ mol/L glutathione.Conclusion. Melatonin can partly inhibit the cytotoxicity of H2O2 and Aβ through its role as a free radical scavenger.
