Molecular epidemiological study on M fragment of fever with thrombocytopenia syndrome virus in parts of China
10.3760/cma/j.cn112866-20200804-00221
- VernacularTitle:中国部分地区发热伴血小板减少综合征病毒M片段分子流行病学研究
- Author:
Shuhan LI
1
;
Zengqiang KOU
;
Zhenhua SHI
;
Xiaorui WANG
;
Hailu ZHANG
;
Xuemin WEI
;
Haowen YUAN
;
Xiaoying XU
;
Jing JI
;
Zhiyu WANG
;
Hongling WEN
Author Information
1. 山东大学齐鲁医学院公共卫生学院微生物检验学系 山东省"十三五"高等学校感染性疾病防控重点实验室，济南 250012
- Keywords:
Severe fever with thrombocytopenia syndrome virus;
Molecular epidemiology;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(6):605-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the basic characteristics and gene evolution of M segment of fever and thrombocytopenia syndrome virus (SFTSV)in China.Methods:The full sequence of M fragment of SFTSV strain isolated in China before June 25, 2020 was obtained from GenBank. The BioEdit software was used for multi-sequence alignment and MEGA5.0 software was used to construct the phylogenetic tree to compare the homology of SFTSV, which came from different types and sources.Results:M fragments of 203 SFTSV isolated in China was collected from GenBank database.. Among them, 185 strains were isolated from humans (91.1%), 11 strains were isolated from ticks(5.4%), and 5 strains were isolated from goat, mice and hedgehog (3.5%). Phylogenetic tree analysis showed that C2 genotype was dominant in China, accounting for 42.4% of total. The nucleotide sequence and amino acid sequence homology were 96.4%~100.0%, 94.5%~100.0%, respectively. The homology of human and animal isolates was very high. The nucleotide sequence and amino acid sequence homology were 92.5%~100.0% and 92.0%~100.0%, respectively.Conclusions:Phylogenetic tree analysis of SFTSV isolates in China based on the M fragment showed that C2 genotype was dominant, and there are multiple types of co-circulation. By comparing M and S-segment-based phylogenetic tree, it suggests that SFTSV may potential undergo adaptive conversion from C-type to J-type.
