Genetic characteristics of rubella virus isolated in Qinghai province
10.3760/cma.j.cn112866-20200806-00222
- VernacularTitle:青海省风疹病毒分离株基因特征分析
- Author:
Chonghai LI
1
;
Chunxiang WANG
;
Shengcang ZHAO
;
Xiaotong WANG
;
Qing ZHOU
;
Zhen ZHU
;
Lixia FAN
Author Information
1. 青海省疾病预防控制中心，西宁 810007
- Keywords:
Qinghai Province;
Rubella virus;
1E genotype;
2B genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(6):616-620
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the genotype and genetic characteristics of rubella virus (RV) circulated in Qinghai province.Methods:The throat swabs were collected from suspected rubella cases in two cities of Qinghai province in 2010 and 2019, respectively. After nucleic acid detection and virus isolation, the target genotyping sequences (739 nucleotide fragments of E1 gene) of positive virus isolates were amplified and determined. The sequences of the viruses were compared with 32 reference strains of 13 genotypes recommended by World Health Organization(WHO) to determine the genotype, and the genetic relationship between Qinghai isolates and RV strains from other provinces of China was also analyzed.Results:In this study, four RV virus strains were isolated, and the genetic relationship analysis showed that these virus strains were classified into 1E-Cluster A sub-genotype and 2B-Cluster C sub-genotype, which was basically consistent with the epidemic trend of RV in other provinces of China. The four virus strains in Qinghai province were highly conserved at the amino acid level, but region-specific mutation sites were also found.Conclusions:This study provides some laboratory data for the formulation of rubella prevention and control strategy in Qinghai province..
