Genetic anglysis of genotype G mumps virus based on its complete genome sequence in Putian city, Fujian province, 2015
10.3760/cma.j.cn112866-20191230-00211
- VernacularTitle:2015年福建省莆田市G基因型腮腺炎病毒全基因组序列测定及分析
- Author:
Hairong ZHANG
1
;
Weiyi PAN
;
Xiuhui YAN
;
Zhifei CHEN
;
Yong ZHOU
;
Dong LI
Author Information
1. 福建省疾病预防控制中心福建省人兽共患病重点实验室，福州 350001
- Keywords:
Mumps virus;
Genome;
Amino acid variation
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(5):485-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the complete genome sequence characteristics of genotype G mumps virus isolated from Putian city, Fujian province in 2015.Methods:Two mumps virus strains isolated in Putian city, Fujian province in 2015 were selected for the study and named as MuV15-01 and MuV15-23.The whole genome of the isolated strains was amplified by reverse transcription-polymerase chain reaction (Rt-PCR). The genetic differences and antigen site variation of the isolated strain were analyzed by comparing the sequences of vaccine strain and other genotypes strains.Results:The total length of genome of MuV isolates was 15, 384 nucleotides. The nucleotide differences between isolates and other genotypes of mumps virus based on the whole genome ranged between 3.7% and 6.0%. The maximum and minimum difference was found between isolates and genotype A (the vaccine strains), and genotype B to N strains, respectively. Among the nucleotide sequences encoding different viral proteins, SH gene had the largest variation, while M and L genes were the most conserved. There were 7 and 11 amino acid mutations in the known antigen related sites of N gene and HN gene, respectively. Compared with the vaccine strain, the isolated strain added an additional N-glycosylation site to the 464 site of the HN gene.Conclusions:Genetic variations were detected in the epidemic mumps virus strains in Putian, Fujian, compared with other mumps genotypes and vaccine strains based on the whole genome. These resluts indicate that the monitoring for genetic differences between epidemic mumps strains and vaccine strains should be further strengthened.
