Whole-genome sequence analysis of human respiratory syncytial virus subgroup A from second-generation sequencing of clinical samples
10.3760/cma.j.cn1128660708-00203
- VernacularTitle:临床样本A亚型人呼吸道合胞病毒二代测序全基因组特征分析
- Author:
Qiong GUO
1
;
Chao WANG
;
Yiman HUANG
;
Qian ZHANG
;
Hao WANG
;
Fenlian MA
;
Lishu ZHENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒与病毒病重点实验室，北京 100052
- Keywords:
Respiratory infections;
Human respiratory syncytial virus;
Whole-genome sequence
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(5):478-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the sequence characteristics and genetic variation of a human respiratory syncytial virus (HRSV) subtype A genome in Beijing.Methods:The genomic RNA of HRSV from nasopharyngeal aspirate samples was sequenced and obtained a whole genome sequence of HRSV A subtype. The phylogenetic tree was constructed with reference sequences of other HRSV strains. The major proteins were compared and single nucleotide polymorphism analyzed. In addition, the N-glycosylationsites of F and G protein were predicted.Results:Phylogenetic tree and homology analysis results suggest that the HRSV strain (RSVA/Beijing-China/2017) was the A subtype ON1 genotype. Nucleotide and amino acid variation analysis showed that G protein, F protein and L protein had some substitutions. Analysis of amino acid variation sites showed that amino acid substitution (L142S) occurred at position 142 of G protein. For F protein, there were two substitutions, which were S105N in the P27 peptide (110-136aa) and C69Y in the antigen sites ? (62-69 aa and 196-210 aa). The prediction of N-glycosylation sites revealed that there were 5 N-glycosylation sites of F protein and 4 N-glycosylation sites of G protein in this strain.Conclusions:The HRSV strain obtained in Beijing belongs to A subtype ON1 genotype. The G, F and L proteins have large variations, and 22 amino acid substitutions have occurred in the G and F proteins.
