Evaluation of a multiplex real-time PCR kit in the detection of viral pathogens in children with acute lower respiratory tract infection
10.3760/cma.j.cn112866-20200220-00026
- VernacularTitle:一种多重呼吸道病毒检测试剂在儿童急性下呼吸道感染病毒病原检测中的初步应用评价
- Author:
Xiangpeng CHEN
1
;
Xiaolei GUAN
;
Yun ZHU
;
Yali DUAN
;
Meng ZHANG
;
Qiuping LI
;
Zhengde XIE
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院 北京市儿科研究所 感染与病毒研究室 儿科重大疾病研究教育部重点实验室 儿童呼吸道感染性疾病研究北京市重点实验室 中国医学科学院儿童危重症感染诊治创新单元，北京 100045
- Keywords:
Children;
Multiplex real-time PCR;
Acute lower respiratory tract infections;
Virus
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):444-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate a multiplex fluorescent probe real-time PCR kit in the viral pathogen detection among children with acute lower respiratory tract infections.Methods:This study used multiplex fluorescent probe real-time PCR method to detect viral pathogens in 300 nasopharyngeal swabs from children with acute lower respiratory tract infection in Beijing Children′s Hospital (influenza A/B virus, respiratory syncytial virus, adenovirus, parainfluenza virus type I and parainfluenza virus type ⅡI), and liquid chip method was used for comparison. In addition, 136 positive-nasopharyngeal aspirates determined by immunofluorescence test were verified by the multiplex fluorescent probe real-time PCR method .Results:For the 300 nasopharyngeal swabs, 173 and 159 were determined positive by real-time PCR and liquid chip kit, respectively. In addition, real-time PCR method found 22 cases of mixed infection samples with two or more viral pathogens compared with the immunofluorescence method .Conclusions:The multiplex fluorescent probe real-time PCR method has high sensitivity and specificity, and has good application value for the detection of children′s acute lower respiratory tract viral pathogens.
