Study on the pathogenesis of HBeAg-positive chronic hepatitis B and plasmacytoid dendritic cells function
10.3760/cma.j.cn112866-20191205-00196
- VernacularTitle:HBeAg阳性慢性乙型肝炎发病与浆细胞样树突状细胞功能的研究
- Author:
Yao LU
1
;
Weihua CAO
;
Qiqi CHEN
;
Huihui LU
;
Lu ZHANG
;
Ge SHEN
;
Shuling WU
;
Hongxiao HAO
;
Min CHANG
;
Ruyu LIU
;
Yuanjiao GAO
;
Leiping HU
;
Minghui LI
;
Yao XIE
Author Information
1. 首都医科大学附属北京地坛医院肝病二科，北京 100015
- Keywords:
Plasmacytoid dendritic cells;
Chronic hepatitis B;
Hepatitis B virus
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):435-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the association between the pathogenesis of hepatitis B e antigen (HBeAg)-positive chronic hepatitis B and the frequency and function of plasmacytoid dendritic cell (pDC) in patients HBeAg-positive chronic hepatitis B virus infection.Methods:A total of 49 HBeAg (+ ) patients with chronic hepatitis B virus infection in immune tolerance phase (IT) and 100 patients in immune clearance phase (IC) were enrolled. The viral serological indicators and liver function were detected. Peripheral venous blood samples were collected. The peripheral blood pDC frequency and the quantitative expression of co-stimulatory molecule CD86 were detected by flow cytometry, and the correlation between the onset of chronic hepatitis B and the frequency and function of pDC was analyzed.Results:In IC group, hepatitis B surface antigen (HBsAg) levels, HBeAg levels and hepatitis B virus (HBV) DNA loads were significantly lower than those in IT group, and alanine aminotransferase (ALT) levels in IC group were significantly higher than that in IT group; pDC% in IC group was significantly lower than that in IT group; CD86 + pDC% and CD86 mean fluorescentintensity (MFI) showed no significant difference between the two groups. In the IC group, the baseline pDC% was negatively correlated with ALT levels, while CD86 + pDC%, CD86MFI, and CD86 antibody binding capacity (ABC) had no remarkable correlation with ALT levels. Conclusions:The frequency of pDC was correlated with the pathogenesis of CHB. The lower the frequency of pDC in patients with CHB, the more prone to hepatitis. Therefore, increasing the frequency of pDC may inhibit the occurrence of hepatitis.
