Evaluation of three laboratory routine methods for inactivating influenza virus
10.3760/cma.j.cn112866-20200610-00186
- VernacularTitle:三种实验室常规方法对流感病毒灭活效果评价
- Author:
Aijun CHEN
1
;
Lishu ZHENG
;
Lihong YAO
;
Chao WANG
;
Qian ZHANG
;
Jianqiang GUO
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会医学病毒与病毒病重点实验室，北京 100052
- Keywords:
UV radiation;
Disinfectant;
Inactivation;
Influenza virus;
Emergency disposal
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):415-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effectiveness of three laboratory routine disinfection methods on influenza virus inactivation.Methods:The physical method of ultraviolet irradiation and two chemical methods of ethanol and "84 disinfectant" were used to inactivate the influenza virus respectively. After disinfecting the virus samples at different times or concentrations, MDCK cells were inoculated and the inactivation effect of influenza virus was identified by observing cytopathic effect (CPE).Results:When the influenza virus was irradiated by ultraviolet light for ≥1 min, treated with 75% ethanol for 0.25-32 min, the effective chlorine concentration of "84 disinfectant" was ≥400 mg/L, and the influenza virus was disinfected at room temperature for ≥1 min, no virus infectivity could be detected under the three conditions.Conclusions:The condition of inactivating influenza virus by routine disinfection methods in laboratory was explored, which provided reference data for establishing the protection specifications and the emergency treatment plans for influenza virus laboratories.
