THE STUDY ON RELATED GENES IN THE NEOPLASTIC TRANSFORMATION OF IMMORTALIZED HUMAN FETAL TRACHEAL FIBROBLAST CELLS INDUCED BY IRRADIATION
- Author:
Meiying YANG
1
;
Leihua LIU
;
Changqing YE
;
Dechang WU
Author Information
1. Institute of Radiation Medicine
- Keywords:
neoplastic transformation;
differential display;
human tracheal fibroblast cells
- From:
Chinese Medical Sciences Journal
1999;14(2):71-74
- CountryChina
- Language:Chinese
-
Abstract:
In this study,we investigated the genes related to the transformation of immortalized human fetal tracheal fibroblast cell line induced by alpha particles by means of differential display Mrna method.The result revealed that there were 23 DNA fragments that were expressed intensively in Αshtf cells(SHTF cells forming clone on agar after irradiated by α particles emitted by 238 Pu)only and not in SHTF(SV40-immortalized human fetal tracheal fibroblast)cells.Northern dot confirmed two fragments,C17-5,C23-1 which showed intensive Mrna expression in Αshtf cells,but not in SHTF cells.The length of the C17-5 fragment was 310bp.Searching in BLAST database revealed that the C17-5 fragment might be an unknown sequence.
