Research on pathogenic spectrum of hand, foot and mouth disease and genetic characteristics of VP1 of Coxsackievirus A6 in Yantai region, Shandong province, China in 2016
10.3760/cma.j.cn112866-20200115-00012
- VernacularTitle:2016年山东省烟台地区手足口病主要病原谱及柯萨奇病毒A6型VP1区基因特征分析
- Author:
Zhenlu SUN
1
;
Xiaodong MOU
;
Hong LIU
;
Ruiqing ZHANG
;
Yingchun XU
;
Lianfeng GONG
;
Juan LIU
;
Qiao GAO
;
ZhaoJing DONG
;
Ji WANG
;
Xuejun MA
Author Information
1. 中国疾病预防控制中心病毒病预防控制研究所 国家卫生健康委员会医学病毒和病毒病重点实验室，北京 102206；烟台市疾病预防控制中心 264003
- Keywords:
Hand, foot and mouth disease (HFMD);
Pathogenic Spectrum;
Coxsackievirus A6(CV-A6);
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):429-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease (HFMD) in the Yantai region of Shandong province in 2016, and analyze the evolution of epidemic strains of coxsackie virus group A type 6 (CV-A6) in the pathogenic spectrum of HFMD enteroviruses and the variations of important amino acid sites in the VP1 region.Methods:A total of 738 samples were collected from the patients with HFMD in Yantai region in 2016 to conduct DNA and serotype tests of enterovirus (EV) by real-time RT-PCR and further count the number and proportion of each type of enterovirus positive specimens. Based on the predominant serotype of enteroviruses, eight serotypes of the CV-A6 strains were selected to carry out VP1 regions amplification for the determination and analysis of nucleotide sequencing and phylogenetic analysis.Results:A total of 460 enteroviruses strains were isolated from 738 samples, including pathogens strains: 258 CV-A16 (56.09%), 62 EV-A71 (13.48%), 49 CV-A10 (10.65%), 44 CV-A6 (9.57%) and 9 CV-A4 (1.96%). Eight CV-A6 positive specimens were isolated from the viruses and the nucleotide-sequence analysis of the whole VP1 region was conducted. The sequence analysis of eight CV-A6 strains demonstrated that the homologies of nucleotide and amino acid were 96.12% - 100% and 97.78% - 100% respectively. The phylogenetic analysis indicated that the eight CV-A6 strains were subdivided into the genotype D subtype D3. Compared with the reference strain, CVA6-Gdula-AY421764, amino acids of CV-A6 strains in Yantai city observed at sites 10, 14, 174, 194, 279, 283 and 305 in VP1 region appeared mutant.Conclusions:CV-A16, EV-A71, CV-A10 and CV-A6 were the main common pathogens of HFMD in Yantai region in 2016. All the CV-A6 strains isolated in this study belonged to subtype D3 in genotype D.
