Effects of methyltransferase like 3 and fat mass and obesity-associated protein on H7N9 virus replication and transcriptome analysis
10.3760/cma.j.cn112866-20200117-00013
- VernacularTitle:甲基转移酶样蛋白3及去甲基化酶肥胖相关蛋白对H7N9病毒复制的影响及转录组学分析
- Author:
Ying LUO
1
;
Ying SUN
;
Hui LIU
;
Bo PENG
;
Weihua WU
;
Xin WANG
;
Yuxuan LEI
;
Qing ZHENG
;
Shisong FANG
Author Information
1. 暨南大学药学院，广州 510632
- Keywords:
Methyltransferase METTL3;
Fat mass and obesity-associated protein (FTO);
H7N9;
Transcriptome analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):385-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and possible molecular mechanisms of m6A methyltransferase METTL3 and demethylase FTO in H7N9 virus infection.Methods:Western blot(WB) was used to detect the expression of METTL3 and FTO after H7N9 virus infected A549 cells. After knocking down and over-expressing METTL3 and FTO, H7N9 virus infection was tested by WB and TCID 50 for its effect on virus replication. Transcriptome sequencing method were used to analyze differentially expressed genes at the transcriptome level in METTL3 or FTO knockdown and wild-type control cells, and preliminary analysis of potential targets was performed. Results:After 24 h of H7N9 virus infection in A549 cells, METTL3 expression was up-regulated, and FTO did not significantly change. After knocking down METTL3 and FTO, the virus nucleoprotein (NP) expression level and virus titer decreased significantly. Consistent with the result of the knockdown experiments, we found that the viral titer was increased by METTL3 and FTO overexpression. Transcriptome sequencing result showed that 314 and 555 differentially expressed genes were found between the METTL3 or FTO knockdown group and the control group, respectively. GO functional enrichment analysis and KEGG pathway enrichment analysis showed that these genes were related to the host immune response.Conclusions:METTL3 and FTO may play a key role in H7N9 virus infection by regulating host-virus interactions.
