Evaluation of virus cultivation in BSL-3 laboratory by basket bioreactor
10.3760/cma.j.cn112866-20200716-00206
- VernacularTitle:应用篮式生物反应器在BSL-3实验室进行病毒培养的评估
- Author:
Wei ZHEN
1
;
Hongyang LIANG
;
Xue ZHAO
;
Zhen CHANG
;
Haiyue SHI
;
Weimin ZHOU
;
Guizhen WU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会生物安全重点实验室，北京 102206
- Keywords:
Biosafety level 3 laboratory;
Basket bioreactor;
Security
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):420-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The risk points of highly pathogenic virus culture in BSL-3 laboratory in basket bioreactor were evaluated, and the safety of virus culture in BSL-3 laboratory was judged by monitoring virus concentration in three stages of virus inoculation, culture and harvest.Methods:Smear samples were taken from the tank and joints of the basket biorereactor at three stages of virus inoculation, culture and harvest with sterilized swabs. At the same time, in the biological safety cabinet, air samples were taken for virus inoculation and harvesting operations using AirPortMD8 air sampler, and in the three stages after operation, the personal protective equipment of researchers was to apply sampling, with three ways to monitoring.Results:During the three stages of virus inoculation, culture and receipt, the virus test result of samples taken in the tank and the junction of the basket bioreactor were all negative, the virus test result of air samples taken during the operation stage of inoculation and receipt were all negative, and the virus test result of samples taken in the personal protective equipment of the experimenter were all negative.Conclusions:The basket bioreactor method for virus culture in BSL-3 laboratory is safe and reliable, and the test result show that there is no virus leakage during the whole operation process, thus ensuring the biosafety of experimenter and laboratory environment.
