Prevalence of human coronaviruses in respiratory infection cases in Beijing from 2016 to 2017
10.3760/cma.j.cn112866-20200109-00006
- VernacularTitle:2016—2017年北京市呼吸道感染病例人冠状病毒流行特征
- Author:
Xiali YU
1
;
Yin FU
;
Yiting WANG
;
Aihua LI
;
Cheng GONG
;
Fang HUANG
Author Information
1. 北京市疾病预防控制中心 北京市预防医学研究中心 100013
- Keywords:
Human coronavirus;
Molecular epidemiology;
Genotype;
Evolutionary analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):396-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the prevalence and gene variation characteristics of human coronavirus (HCoV) in Beijing and provide molecular epidemiological basis for the prevention and control of HCoV infection.Methods:This study collected 287 samples and epidemiological data of HCoV positive cases collected by respiratory multi-pathogen surveillance in Beijing from 2016 to 2017, and analyzed the HCoV detection rate, population and seasonal characteristics. HCoV pol gene fragments were amplified by RT-PCR for HCoV typing and evolutionary analysis.Results:In this study, the detection rate of HCoV was 1.89%. The co-infection rate of respiratory virus in HCoV positive samples was 20.56% (59/287). 69 strains were identified as type OC43 with nucleotide homology of 97.2%~100% and amino acid homology of 92.2%~100%. Thirty-nine strains were identified as type 229E with nucleotide homology of 99.2%~100% and amino acid homology of 99.2%~100%. Ten strains were identified as type HKU1 and 2 strains were identified as type NL63.Conclusions:OC43 and 229E are still the main types of HCoV in Beijing from 2016 to 2017, and both present certain seasonal difference. The endemic strains of the same type have high homology of nucleotide and amino acid.
