Prevalence of hand, foot and mouth disease associated coxsackievirus A10 and analysis of VP1 gene variation in Anyang city, Henan province
10.3760/cma.j.cn112866-20200628-00197
- VernacularTitle:河南省安阳市手足口病相关柯萨奇病毒A10流行状况及VP1基因变异分析
- Author:
Guoqing SUN
1
;
Yang LI
;
Xiangping ZHANG
;
Nan XIE
;
Long LIU
Author Information
1. 河南省疾病预防控制中心，郑州 450016
- Keywords:
Hand, foot and mouth disease;
Coxsackie virus A10;
Gene amplification;
Analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):424-428
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the epidemic situation of coxsackievirus A10 (CV-A10) hand, foot and mouth disease (HFMD) in Anyang city, Henan province and analyze the variation and evolution characteristics of VP1 gene, so as to provide the basis for the research of CV-A10 virus and the prevention and control of HFMD.Methods:From 2011 to 2015, HFMD cases were enrolled in the study. epidemiological information and fecal samples were obtained. Fluorescent quantitative PCR was used to detect and type enterovirus. The CV-A10 VP1 gene was amplified and sequenced, and the subtype was identified. Phylogenetic tree was constructed to analyze the evolution and variation characteristics of the virus.Results:A total of 2270 fecal samples (from 2011 to 2015, 277, 514, 479, 505 and 495 per year, separately). Totally 1863 enteroviruses were detected, the positive rate was 82.07%. CV-A16, EV-A71, CV-A6 and CV-A10 accounted for 94.26% of all enteroviruses. Based on all of VP1 gene sequences of CV-A10 downloaded from database, four genotypes A, B, C and D were identified. The nucleotide sequence similarity was 75.1%-84.2%. Two subtypes of CV-A10 were identified in this area.Conclusions:CV-A16 and EV-A71 are the dominant viruses causing HFMD, but attention should also be paid to the pathogens of CV-A6 and CV-A10. Although the prevalence of CV-A10 has kept stable in recent years, but there is still a risk of rising of its prevalence.
