Bioinformatics analysis of the mechanism of lung injury and intervention drugs associated with SARS coronavirus infection
10.3760/cma.j.cn112866-20200318-00074
- VernacularTitle:严重急性呼吸综合征冠状病毒感染肺损伤机制与干预药物的生物信息学分析
- Author:
Jundong ZHANG
1
;
Bo YANG
;
Lin LIU
;
Haoran CHEN
;
HaoMin ZHANG
;
Bin GUO
;
Ximeng CHEN
;
Peng ZHI
;
Zhuoyang LI
;
Geliang LIU
;
Xiaohua CHI
;
Xuechun LU
Author Information
1. 解放军医学院，北京 100853
- Keywords:
Coronavirus;
Lung Injury;
Bioinformatics;
Drug repositioning
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):367-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the mechanism and potential intervention drugs of acute lung injury caused by severe acute respiratory syndrome coronavirus (SARS-CoV) infection in order to provide reference for the treatment of COVID-19.Methods:Gene Expression Omnibus (GEO) announcement database was used to screen coronavirus transcriptome data, and R language package was used for differential expression analysis and Kyoto Gene and Genome Encyclopedia (KEGG) and Gene Ontology (GO) enrichment analysis. A protein-protein interaction (PPI) network analysis was carried out by using STRING online analysis website, and the key genes were screened. Then the Epigenomic Precision Medicine Prediction Platform (EpiMed) was used to analyze the association of key genes and predict potential therapeutic drugs.Results:Based on the whole genome expression profile data of SARS-CoV, a total of 3 606 differential genes were screened, including 2 148 up-regulated and 1 458 down-regulated. GO enrichment is mainly related to viral infection, leukocyte migration and adhesion, acute inflammation and collagen secretion. KEGG enrichment is mainly related to signal transduction, acute inflammation, immune response and so on. Ten key genes related to lung injury, such as PTPRC, TIMP1, ICAM1 and IL1B, were screened by protein interaction network analysis. EpiMed platform predicted that pulsatilla chinensis, polygonum cuspidatum, tumor necrosis factor-α inhibitors, famciclovir, fluvastatin and other drugs have potential therapeutic effects.Conclusions:SARS-CoV infection can cause lung injury by activating a series of inflammation-related molecules. Drugs that may be effective in the treatment of coronavirus infections, including pulsatilla chinensis, polygonum cuspidatum, tumor necrosis factor-α inhibitors, famciclovir and fluvastatin.
