Comparison of mariPOC ? multiplex assay and direct immunofluorescence assays for detection of respiratory virus in children with pneumonia
10.3760/cma.j.cn112866-20200309-00052
- VernacularTitle:mariPOC ?多重分析法与直接免疫荧光法检测肺炎患儿感染呼吸道病毒结果的比较
- Author:
Dali WANG
1
;
Yue LI
;
Qi ZHENG
;
Yuning CHE
;
Chao XU
;
Zhenze CUI
Author Information
1. 大连市儿童医院中心实验室 116012
- Keywords:
Rapid antigen testing;
Respiratory tract virus;
MariPOC ? multianalyte assay;
Pneumonia
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(3):304-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective:mariPOC ? multianalyte assays and direct immunofluorescence assays (DFA) were used to detect respiratory viruses in children with pneumonia and evaluate the performance of mariPOC ? method by comparing the results. Methods:Nasopharyngeal swab samples were tested by both mariPOC ? and DFA for influenza A and B, respiratory syncytial viruse, adenoviruse, and parainfluenza 1, 2, and 3 viruses. Real-time PCR was also used to detect the DNA of FluA、FluB、RSV、PIV 1、2、3 and ADV as reference method, in order to verify the inconsistent result of the above two method. Results:Four hundred children with pneumonia were enrolled. The total positive rates of mariPOC ? and DFA were 40.25% and 38.25% respectively. According to the results, the positive consistency between mariPOC ? and DFA was 92.16% and the negative consistency was 91.90%, the overall agreement rate was 92.00%. There were 32 samples where mariPOC ? and DFA gave contradicting results. In real-time PCR confirmation, the result of 20 samples were consistent with mariPOC ? test and 12 agreed with DFA. Compared with real-time PCR, the sensitivity of mariPOC ? was 93.06%, the specificity was 100.00%, and the total coincidence rate was 97.00%. Conclusions:mariPOC ? multianalyte assays had good correlation with DFA for detection of respiratory viruses in children with pneumonia. Compared with the reference method real-time PCR, it has better sensitivity, specificity and total coincidence rate. mariPOC ? multianalyte assay has the obvious advantages of high sensitivity, fast detection and intelligence, which can be effectively applied to clinical examinations.
