Expression and receptor binding characteristics of horse P12 genotype rotavirus GST-VP8*-E403 protein
10.3760/cma.j.cn112866-20200409-00119
- VernacularTitle:马源P[12]型轮状病毒GST-VP8*-E403蛋白的表达及受体结合特征
- Author:
Mengxuan WANG
1
;
Jiayan ZHANG
;
Qiuyan ZENG
;
Xiaoman SUN
;
Qing ZHANG
;
Hong WANG
;
Dandi LI
;
Zhaojun DUAN
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，北京 102206
- Keywords:
Rotavirus;
VP8* protein;
Glycan;
Receptor
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(3):309-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the binding characteristics of horse-derived P[12] rotavirus GST-VP8*-Horse P[12]E403 protein to oligosaccharides and saliva receptors, provides an important scientific basis for the cross-species transmission and the mechanism of interaction between the bodies.Methods:The E. coli expression system was used to express and purify the horse-derived P[12] rotavirus GST-VP8*-Horse P[12]E403 protein. The receptor binding characteristics of this genotype were analyzed by saliva and oligosaccharide binding experiments. Results:Horse-derived GST-VP8*-Horse P[12]E403 protein binds well with mucin core 2 sugar, but does not bind to other oligosaccharides such as A, B, Lewis, and HBGAs in saliva.Conclusions:The potential receptor of VP8*-Horse P[12]E403 protein may be mucin core 2, and it did not bind to human saliva.
