Analysis of the genetic characterization and circulation dynamic of echovirus 11 based on environmental sewage surveillance in Shanghai, China
10.3760/cma.j.cn112866-20191204-00195
- VernacularTitle:上海市环境污水监测埃可病毒11型基因特征和循环动态
- Author:
Yunyi LI
1
;
Jing LU
;
Yuying YANG
;
Jie FEI
;
Liping ZHANG
;
Zhi LI
;
Jiayu HU
;
Chongshan LI
Author Information
1. 上海市疾病预防控制中心 200336
- Keywords:
Echovirus 11;
Surveillance;
Genetic characterization;
Circulation
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(3):270-275
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore genetic characterization and circulation dynamic changes of Echovirus 11(Echo11)isolated from environmental sewage surveillance.Methods:The VP1 coding region of Echo11 isolated from environmental surveillance in Shanghai from 2012-2019 was amplified and sequenced. Homology analysis and phylogenetic analysis on the Echo11 VP1 sequences were performed with reference strains in acute flaccid paralysis (AFP) surveillance, healthy children surveillance and reference strains.Results:A total of 156 strains of Echo11 were isolated from different sources in Shanghai, of which 136 strains were isolated from environmental sewage in 2012-2019, with the seasonal peak mainly in summer and autumn; 40.3% of the strains from different sources in Shanghai had the 54th amino acid mutation. There are 5 subgenotype of Echo11 from different sources in Shanghai, i. e. A1, A2, C1, C3 and D5. During 2012, there was co-circulation of A1 subgenotype Echo11 among environmental sewage, AFP cases and healthy children. From 2012 to 2015, the dominant transmission chain of Echo11 was A1 subgenotype. Since 2016, the dominant transmission chain of echo11 has gradually changed to D5 subgenotype.Conclusions:Environmental surveillance truly reflects the circulation of human enterovirus(HEV), which has practical significance for the prediction and early warning of HEV related diseases.
