Application of multiple RT-PCR assay for respiratory system infection in elderly patients
10.3760/cma.j.cn112866-20200414-00125
- VernacularTitle:呼吸道病原体多重核酸扩增在老年患者呼吸系统感染检测中的应用
- Author:
Guowei SONG
1
;
Jing PAN
;
Yun LIANG
;
Xuejun MA
;
Lingling WAN
;
Yuxue LI
;
Lixin LI
Author Information
1. 石家庄市第一医院检验科 050000
- Keywords:
Respiratory tract infection;
Capillary electrophoresis;
Elderly patients;
Reverse transcription polymerase chain reaction
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(3):299-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand application value of pathogen detection in elderly patients with respiratory infection by using multiple RT-PCR to detect 13 common pathogens of respiratory tract infection.Methods:This is a retrospective study, which included 317 elderly patients, aged 60 to 98 years with respiratory symptoms, who visited Geriatrics of the First Hospital of Shijiazhuang between June 2016 and May 2017. Sputum of each patient was collected. After the viral DNA/RNA was extracted, 13 respiratory pathogens were detected by multiplex RT-PCR and capillary electrophoresis. The clinical performance of the assay was evaluated in comparison to direct sequencing and Real-time PCR.Results:Compared with the direct sequencing and Real-time PCR, the accuracy of multiple RT-PCR was 99.93% (95% CI: 99.79%-99.98%), positive predictive value was 99.24% (95% CI: 97.78%-99.74%), negative predictive value was 100% (95% CI: 99.9%-100%), and Cohen’s kappa was 0.9958 (95% CI: 0.9652-1.026). Among the 317 elderly patients, the positive rate of respiratory infection was 75.7% (240/317). The highest was influenza A, 20.2% (64/317), mainly H3N2, 70.3% (45/64), followed by rhinovirus, adenovirus, Mycoplasma pneumoniae and influenza B, with the positive rates 15.5% (49/317), 13.6% (43/317), 11.0% (35/317) and 10.1% (32/317), respectively. In addition, 81 (25.6%) elderly patients were infected with two or more pathogens. Conclusions:Multiple RT-PCR meet the needs of clinical detection of respiratory pathogens and provide information for the prevention and treatment of respiratory system Infection.
