Molecular etiological characteristics of the gastroenteritis outbreak associated with Norovirus infection in Deyang city, 2018

VernacularTitle:2018年德阳市胃肠炎暴发中诺如病毒分子病原学特征研究
Author: Ranran CAO ¹ ; Yang HE ; Xiaoyu WU ; Xiaozhen MA ; Min REN
Author Information

1. 四川大学，成都　610041；四川省疾病预防控制中心，成都　610041
Keywords: Norovirus; Gastroenteritis; Outbreak; Phylogenetic analysis
From: Chinese Journal of Experimental and Clinical Virology 2020;34(3):284-288
CountryChina
Language:Chinese
Abstract: Objective:To investigate the genotype and genetic characteristics of the pathogens associated with the epidemic outbreak of acute gastroenteritis (AGE) in Deyang city, Sichuan province, and to provide perspectives on disease prevention and vaccine development.Methods:Norovirus (NoV) nucleic acid in the sixty-three samples collected from three AGE outbreaks were detected by Real-time RT-PCR. The genes of RNA-dependent RNA polymerase (RdRp) and capsid protein (VP1) of the positive samples were amplified by conventional RT-PCR and sequenced and made phylogenetic analysis.Results:Three AGE outbreaks were all caused by NoV, and twenty-one samples were positive for NoV GII. Two of the outbreaks were caused by GII.17[P17] NoV and shared high similarities with global reference sequences from 2014. One of the outbreaks was caused by GII.2[P16] NoV and shared high similarities with global reference sequences from 2016.Conclusions:The three AGE outbreaks in Deyang city in 2018 were caused by two different NoV genotypes.