Application of multiplex real-time PCR assay and TaqMan array cards in the detection for diagnosing acute respiratory viral infections in children
10.3760/cma.j.cn112866-20190906-00139
- VernacularTitle:多重PCR技术和呼吸道病原体微流体芯片在诊断儿童急性呼吸道病毒感染中的应用
- Author:
Jianan XU
1
;
Ming PAN
;
Tianshu LI
;
Haiyan WU
;
Wenbin TONG
;
Shusen HE
;
Huiping YANG
Author Information
1. 四川省疾病预防控制中心微生物检验所，成都 610041
- Keywords:
Respiratory tract infection;
Multiplex PCR;
TaqMan array cards;
Rapid detection
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(2):202-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the diagnostic value of multiplex PCR (RespiFinder?SMART 22 test kits) and respiratory pathogen screening microfluid chip (TaqMan array cards, TAC) in acute respiratory infection (ARI) in children.Methods:A total of 275 respiratory samples were collected from the Chengdu Children′s Specialist Hospital with respiratory symptoms between August 2018 and August 2019. We randomly selected 120 samples from these respiratory samples. Virus nucleic acid detection of 120 samples were performed with the RespiFinder? SMART 22 and TAC, respectively. And then, we compared the advantages and disadvantages of the two different detection methods.Results:One hundred and twenty samples were detected by RespiFinder?SMART22 and TAC, the positive rates were 88.33% and 93.33%, respectively. The mixed infection rate was 26.67% and 35%, respectively. Among the 120 samples, the consistency rate of the two methods was 88.33%, of which the positive concordance was found in106 samples and the negative concordance was in 7 samples. For 7 cases the result were discordant.Conclusions:In this experimental test, multiplex PCR and TAC have a high consistency in the positive samples, both of which have good clinical application value for the detection of respiratory virus.
