Discovery and etiological diagnosis of the first imported Chikungunya fever epidemic in Hunan province
10.3760/cma.j.cn112866-20191121-00185
- VernacularTitle:湖南省首起输入性基孔肯雅热疫情的发现与病原学诊断
- Author:
Fangling HE
1
;
Hengjiao ZHANG
;
Juan WANG
;
Jian HE
;
Xuxiang LONG
;
Bei LIU
;
Rui YANG
;
Shixiong HU
;
Zhifei ZHAN
;
Liang CAI
;
Lidong GAO
Author Information
1. 湖南省疾病预防控制中心，长沙 410005
- Keywords:
Chikungunya fever;
Chikungunya virus;
Phylogenetic tree;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(2):175-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To confirm the first imported Chikungunya fever (CHIK) epidemic in Hunan province.Methods:Serum samples of patients and colleagues were collected. The nucleic acids of Dengue virus (DENV), Yellow fever virus (YFV), Chikungunya virus (CHIKV) were detected by real- time fluorescent quantitative PCR. The positive PCR products were sequenced. Phylogenetic tree was constructed.Results:The serum samples of the patient and one of the five colleagues were positive for CHIKV. The Blast comparison of gene sequence showed 99% homology with CHIKV sequences. The infected CHIKV belonged to ECSA genotype in the phylogenetic tree.Conclusions:The first imported CHIK epidemic in Hunan province was confirmed through the epidemiological survey and etiologic detection.
