Investigation of norovirus and rotavirus contamination in ball lettuce sold in Beijing
10.3760/cma.j.cn112866-20191031-00169
- VernacularTitle:北京市市售结球生菜诺如病毒和轮状病毒污染状况调查
- Author:
Hanqiu YAN
1
;
Yanwei CHEN
;
Yi TIAN
;
Weihong LI
;
Baiwei LIU
;
Zhiyong GAO
;
Quanyi WANG
Author Information
1. 北京市疾病预防控制中心传染病地方病控制所 北京市预防医学研究中心 食物中毒诊断溯源技术北京市重点实验室 100013
- Keywords:
Ball lettuce;
Norovirus;
Rotavirus;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(2):151-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the contamination of ball lettuce sold by norovirus and rotavirus in Beijing.Methods:From November 2015 to October 2016, 54 samples were collected at a market stall in Beijing. The potentially existing viruses were eluted and concentrated from the ball lettuce leaves using centrifugation and direct methods corresponding to three kinds of eluants. Norovirus and rotavirus were detected by real time RT-PCR. The partial VP1 gene of norovirus was amplified by semi-nested RT-PCR. The PCR product was sequenced directly, the sequence alignment was performed using the BioEdit 7.0.9.0 software, the phylogenetic tree was constructed with the MEGA 6.06 software.Results:Among 54 pieces of ball lettuce, rotavirus was detected negative, and the detection rate of norovirus was 11.11% (6/54). Of these NoV-positive specimens, 3 (50.0%) were NoV GII, 1 (16.7%) was NoV GI, and 2 (33.33%) were a mix of NoV GI and GII, The detection rates in spring, summer, autumn and winter were 8.3% (1/12), 0.0% (0/8), 28.6% (4/14) and 5.0% (1/20), respectively. One strain was sequenced successfully and identified as GII.3 genotype. The strain shares 100.0% nucleotide identity with strain KY348698 isolated from human in Guangdong in 2014.Conclusions:Human norovirus contamination existed in some ball lettuce sold in Beijing, eating raw unwashed ball lettuce may indicate a risk of viral acute gastroenteritis.
