Clinical study on dysbiosis of intestinal microbiota in patients with inflammatory bowel disease
10.3760/cma.j.cn112866-20191203-00192
- VernacularTitle:炎症性肠病患者肠道菌群的临床研究
- Author:
Li TIAN
1
;
Jiegang HUANG
;
Hao LIANG
;
Li YE
;
Ping CUI
;
Xiaodan ZHONG
;
Yu ZHANG
;
Jie ZHOU
;
Hui CHEN
Author Information
1. 广西医科大学第一附属医院，南宁 530000
- Keywords:
Inflammatory bowel disease;
Crohn′s disease;
Ulcerative Colitis;
Intestinal microbiota;
16s rRNA sequence
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(2):180-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To describe the composition of fecal microbiota in patients with inflammatory bowel disease, identify the bacteria related to inflammatory bowel disease, and to provide new ideas for the treatment and diagnosis of the disease.Methods:Fecal samples were obtained from patients with Crohn’s disease (CD, n=20), ulcerative colitis (UC, n=10) and healthy controls ( n=30). A 16S rRNA sequencing approach was applied to determine the compositions of microbial communities in fecal samples. Results:The microbial structure of patients with inflammatory bowel disease was significantly different from that of the control group. Abundance of Proteobacteria, Epsilonbacteraeota, Acidobacteria, Escherichia-Shigella were significantly higher in IBD patients relative to healthy controls, whereas significantly lower abundances were observed for Prevotella_9, Megamonas and Lachnoclostridium. The abundance of Lachnoclostridium in Crohn′s disease was significantly higher than that in ulcerative colitis, while Acidobacteria and Prevotella _9 was significantly lower than that in ulcerative colitis. The abundance of Firmicutes and Bacteroidetes were decreased in disease cohorts, but the difference was not statistically significant ( P>0.05). Conclusions:Intestinal microbial disorders in patients with IBD, and increased pathogenic bacteria (mainly proteobacteria and shigella Escherichia coli) in the intestinal microflora of IBD patients. The difference in microbiota between Crohn′s disease and ulcerative colitis may be related to the disease.
