Isolation, identification and genetic evolution analysis of Coxsackievirus A group 2 type
10.3760/cma.j.cn112866-20190729-00119
- VernacularTitle:柯萨奇病毒A组2型的分离鉴定与遗传进化分析
- Author:
Ziwei LIU
1
;
Chunxia LI
;
Jing JI
;
Shaoxia SONG
;
Li ZHAO
;
Zhiyu WANG
;
Hongling WEN
Author Information
1. 山东大学公共卫生学院微生物检验学系 感染性疾病防控重点实验室(山东省"十三五"高校重点实验室)，济南 250014
- Keywords:
Coxsackievirus A group 2;
Hand, foot and mouth disease;
Virus isolation;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(2):160-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify the virus from stool samples of children with hand, foot and mouth disease (HFMD) in Linyi City, Shandong Province in 2017, and analyze its genetic characteristics.Methods:Nucleic acid was detected in fecal specimens of children with hand, foot and mouth disease. Human rhabdomyosarcoma cells were used for virus isolation. The whole genome of the virus was sequenced, and the phylogenetic analysis and gene recombination analysis of the isolates were carried out by comparing with human enterovirus.Results:A coxsackievirus A group 2 type(CoV-A2), named SD17-430, was successfully isolated from fecal specimens of children with severe HFMD. Phylogenetic analysis further confirmed that the coxsackievirus genotype belonged to D2 genotype. The coding region of SD17-430 capsid protein had high homology with CoV-A2 international original strain, while the coding region of non-structural protein had high homology with CoV-A4 (MH086949) and CoV-A14 (KP036482).Conclusions:Compared with the original strain, CoV-A2 SD17-430 strain has a greater degree of genetic variation, and may have genetic recombination with multiple enteroviruses during its evolution. We should continue to strengthen the overall monitoring of HFMD pathogens in order to further understand the changes of pathogen spectrum and provide data reference for formulating more effective strategies for HFMD prevention and control.
