Characteristic spectrum and ginseng content determination of the famous classical formula Fuzi decoction
10.3969/j.issn.1006-2157.2024.06.018
- VernacularTitle:经典名方附子汤特征图谱及其人参含量测定研究
- Author:
Xueyi WANG
1
,
2
;
Junshuai WANG
;
Zhenxia ZHAO
;
Zhenyin LIU
;
Xueying WANG
;
Bing XU
;
Jian SU
;
Yongli LIU
Author Information
1. 河南大学药学院 开封 475004
2. 河北省药品医疗器械检验研究院 河北省中药质量评价与标准研究重点实验室
- Keywords:
Fuzi decoction;
famous classical formula;
substance benchmark;
characteristic chromatogram;
content determination
- From:
Journal of Beijing University of Traditional Chinese Medicine
2024;47(6):863-873
- CountryChina
- Language:Chinese
-
Abstract:
Objective We aimed to establish the benchmark characteristic map of Fuzi decoction and the determination method of multi-index components,and to clarify the key quality attributes of Fuzi decoction.Methods Fifteen batches of Fuzi decoction substance benchmarks samples were prepared;the high-performance liquid chromatography(HPLC)characteristic spectrum of Fuzi decoction was established;and characteristic peak attribution and similarity analysis were performed.Ginsenosides Rg1,Re,Rf,and Rb1 were used as the index components to establish a method to determine ginseng content in Fuzi decoction.The value range of each quality control index was set at a limit of 70%-130%of the average value,and the quantity transfer analysis was performed on the material basis of 15 Fuzi decoction batches.Results The characteristic spectra of the 15 Fuzi decoction batches had 12 common peaks,and seven characteristic peaks of gallic acid,catechin,paeoniflorin,ginsenosides Rg1,Re,and Rb1,and atractylenolide Ⅲ were identified,with a similarity of more than 0.98.Ginsenosides Rg1,Re,Rf,and Rb1 content ranges were 0.51-0.94,0.34-0.62,0.14-0.27,and 0.41-0.76 mg/g,respectively.The transfer rates of ginsenosides Rg1 and Re,Rf,and Rb1 were 12.05%-26.91%,11.15%-43.71%,and 10.53%-33.23%,respectively.Conclusion The characteristic HPLC of Fuzi decoction and the determination method of Fuzi decoction ginseng content established in this study are accurate,reproducible,and stable,laying the foundation for the quality evaluation of the key chemical properties of Fuzi decoction and its preparation.
