Comparison of three nucleic acid detection methods for hepatitis E virus
10.3760/cma.j.issn.1003-9279.2020.01.014
- VernacularTitle:戊型肝炎病毒3种核酸检测方法的比较
- Author:
Wenjiao YIN
1
;
Feng QIU
;
Wenting ZHOU
;
Jingyuan CAO
;
Hongtu LIU
;
Shengli BI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所国家卫生健康委员会医学病毒和病毒病重点实验室
- Keywords:
Hepatitis E virus;
Quantitative real-time polymerase chain reaction;
Genotyping
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(1):67-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the performance of three nucleic acid detection methods for hepatitis E virus.Methods The open reading frame (ORF) 2 gene sequence of HEV genotype 4 representative strain was cloned into pUC57 vector.Plasmid DNA was detected by two real-time quantitative method A and B,and the detection limits were compared.Other samples were used for specificity detection.Serum specimens of acute hepatitis E patients were detected by three method,and the results were compared.Results The lowest detection limit of plasmid DNA by A and B method can both reach 35 copies/reaction,with specificity of 100％.The HEV RNA positive rate of serum samples from acute hepatitis E patients by A,B and C method was 47.8％ (43/90),43.3％ (39/90) and 41.1％ (37/90),with the concordance rate of 88.9％ (80/90).There was no statistically significant difference among the three method (x2=0.8414,P=0.6566).Serum specimens with Ct values below 34.6 detected by method A,or below 35.6 detected by method B,the success rate of amplification by method C was 100％.Conclusions Method A has both higher sensitivity and specificity,and method C is a sensitive gene detection method.
