Prevalence of norovirus infection in 8 outbreaks of infectious diarrhea in Huzhou city, 2018

VernacularTitle:2018年湖州市8起腹泻聚集疫情中诺如病毒的感染与流行
Author: Xiaofang WU ¹ ; Lei JI ; Guangtao LIU ; Deshun XU
Author Information

1. 湖州市疾病预防控制中心 313000
Keywords: Norovirus; Diarrhea; Outbreak; Prevalence
From: Chinese Journal of Experimental and Clinical Virology 2020;34(1):38-42
CountryChina
Language:Chinese
Abstract: Objective To analyze the genetic characteristics of norovirus (NoV) outbreaks in Huzhou city in 2018.Methods Totally 73 stool samples were collected from NoV outbreaks and tested by real-time RT-PCR.The polymerase and capsid protein regions were amplified from the norovirus nucleic acid positive samples in each cluster epidemic.The amplified products were further analyzed by gene sequencing and genotyping.Results A total of 57 specimens from 8 outbreaks were positive for GII type NoV nucleic acid and 5 samples were positive for both genogroup Ⅰ and genogroup [I.The alignment of polymerase and capsid protein nucleic acid sequences showed that 5 cases were GII.P16/GII.2 type,1 case was GII.P17/ GII.17 type,1 case was GII.P12/GII.3 type,and 1 case was mixed infection with GI.P4/GI.5 type and GII.P17/GII.17 type.Conclusions NoV is the main pathogen causing the epidemic of acute gastroenteritis and diarrhea in Huzhou city.The virus was mainly GII type,with multiple genotypes and recombinant strains.