Molecular characterization of envelope gene of dengue virus type 2 imported from Bangladesh to Wuhu, Anhui provice, China
10.3760/cma.j.issn.1003-9279.2020.01.007
- VernacularTitle:孟加拉国输入芜湖2型登革病毒E基因分子特征
- Author:
Gongfei WANG
1
;
Zhou AN
;
Zhouxiang CHENG
;
Yongling SHI
;
Bin SU
Author Information
1. 芜湖市疾病预防控制中心 241000
- Keywords:
Dengue virus;
E gene;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the genotype of dengue virus (DENV) and its molecular characteristics of E gene from 1 case imported from Bangladesh to Wuhu city.Methods The viral RNA was extracted from patient serum sample,the serotype of DENV was determined by Real-time RT-PCR through screening the universal and serotypes 1/2/3/4 of DENV.The completed sequence of DENV E gene was amplified,the phylogenetic tree of E gene was constructed by MEGA 7.0 software,and the nucleotide and amino acid sequences alignments of E gene were analyzed.Results Patient serum was positive for DENV type 2 nucleic acid.The DENV type 2 belonged to the Cosmopolitan genotype by phylogenetic analysis of the E gene,and it had the highest homology with the isolate D2/Malaysia/1408aTw which was imported from Malaysia to Taiwan in 2014,the homologies of nucleotide and amino acid reached 99.60％ and 99.80％,respectively.Totally 6 nucleotides (42:G→A;141:G→A;490:G→A;516:A→G;954:A→G;1344:C→ T) and 1 amino acid (164:V→I) had changed in E gene.E protein possessed attenuated strain characteristic site E126-E and virulent strain characteristic site E383-385-E-P-G.Conclusions The imported patient is infected with DENV type 2 and the virus is originated from Malaysia.
