Inactivation effect of heat and common three chemical disinfectants on enterovirus A 71
10.3760/cma.j.issn.1003-9279.2020.01.004
- VernacularTitle:肠道病毒A组71型热力和常见3种化学消毒剂灭活效果的研究
- Author:
Jie LI
1
;
Dongmei YAN
;
Yong ZHANG
;
Wenbo XU
;
Shuangli ZHU
;
Weimin ZHOU
;
Guizhen WU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委员会生物安全重点实验室
- Keywords:
Enterovirus A71;
Biosafety of laboratory;
Public health
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(1):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the inactivation effect of heat inactivation and three common chemical disinfectants on enterovirus group A 71 (EV-A71),provide basic data for disinfection work after laboratory activities in enterovirus secondary biosafety laboratory.Methods EV-A71 virus was treated at different temperatures and different disinfectant concentrations according to different time.The inactivated virus was inoculated into 96-well cell culture plates,and the cytopathic effect was observed and recorded.Finally,the virus titer of the virus treatment solution was calculated.Results After inactivation at 56 ℃ for 30 min,the inactivation logarithm of EV-A71 was more than 6,and inactivation at 65 ℃ for 30 rin,70 ℃ for 10 min,no infection was observed.Three chemical disinfectants could effectively inactivate a certain titer of EV-A71 at a certain concentration and treatment time.Conclusions The inactivation effect of EV-A71 virus is related to factors such as thermal inactivation temperature,chemical disinfectant concentration,and treatment time.Studying the inactivation curve of EV-A71 is beneficial to the selection of inactivation conditions in different inactivation environments.
