Analysis of serum metabolomic characteristics of patients in the active stage of ulcerative colitis with syndrome of dampness-heat in large intestine
10.3969/j.issn.1006-2157.2024.05.013
- VernacularTitle:溃疡性结肠炎活动期大肠湿热证患者血清代谢组学特征分析
- Author:
Changchang GE
1
,
2
;
Yi LU
;
Hong SHEN
;
Lei ZHU
Author Information
1. 南京中医药大学附属医院 南京 210029
2. 连云港市第一人民医院
- Keywords:
ulcerative colitis;
syndrome of dampness-heat in large intestine;
metabolomics;
modernization of traditional Chinese medicine
- From:
Journal of Beijing University of Traditional Chinese Medicine
2024;47(5):686-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the metabolomics characteristics of patients in the active stage of ulcerative colitis(UC)with syndrome of dampness-heat in large intestine through non-target metabolomics technology,and to provide a basis for promoting the theoretical system of traditional Chinese medicine(TCM)syndrome differentiation of disease syndrome combination and micro-macro combinations.Methods Non-target metabolomics technology was used to detect the serum samples from 159 patients in the active stage of UC(81 cases with syndrome of dampness-heat in large intestine and 78 cases with syndrome of non-dampness-heat in large intestine)and 30 healthy volunteers.The orthogonal partial sample least squares discriminant analysis model was constructed to screen metabolites with significant changes among groups.The variable importance in projection≥1,P<0.05,and fold change(FC)>1.20 or FC<0.83 were used as the criteria for the screening of differential metabolites.The Kyoto Encyclopedia of Genes and Genomes(KEGG)was used to annotate differential metabolites,and MetaboAnalyst software was used for pathway analysis.Results Between patients in active stage of UC with syndrome of dampness-heat in large intestine and syndrome of non-dampness-heat in large intestine,a total of 99 differential metabolites were screened in the positive ion mode,of which 48 were upregulated and 51 were downregulated.In the negative ion mode,a total of 38 differential metabolites were screened,of which 19 were upregulated and 19 were downregulated.The KEGG enrichment analysis showed that there were 21 metabolic pathways,and the pathway analysis showed that there were mainly four metabolic pathways involved in tryptophan metabolism,sphingolipid metabolism,glycerophospholipid metabolism,and pyrimidine metabolism.Conclusion Patients in the active stage of UC with syndrome of dampness-heat in large intestine have abnormal metabolic pathways,which can provide a basis for TCM syndrome differentiation and treatment for UC with syndrome of dampness-heat in large intestine.
