Characterization of norovirus in adults with acute gastroenteritis in Shunyi district of Beijing from 2014 to 2015
10.3760/cma.j.issn.1003-9279.2016.06.007
- VernacularTitle:2014-2015年北京顺义急性胃肠炎成年患者中诺如病毒分子进化规律分析
- Author:
Shuang ZHANG
1
;
Hongbo JING
;
Hongmei MA
;
Miao JIN
;
Ying LI
Author Information
1. 101300,北京市顺义区疾病预防控制中心
- Keywords:
Norovirus;
Acute gastroenteritis;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(6):526-531
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the infection and genetic characteristics of norovirus in adults with acute gastroenteritis in Shunyi district of Beijing.Methods From January 2014 to December 2015,stool specimens and clinical data were collected from 240 adults with acute gastroenteritis from enteric clinics in Shunyi district of Beijing.Norovirus was detected by Real-time PCR.For norovirus-positive samples,reverse transcription-PCR (RT-PCR) was performed to amplify RNA-dependent RNA polymerase(RdRp) gene fragment.The RdRp regions of norovirus-positive samples were sequenced and performed genotype analysis.Results The positive rate of norovirus was 23.33％ (56/240).G Ⅱ group was the predominant genogroup (20.83％,50/240),followed by G Ⅰ group (2.50％,6/240).There was no significant difference in the norovirus positive rate between male and female (x2 =0.21,P =0.65).Norovirus could be detected throughout the year,and the prevalent peak was between November and March of the following year,from 2014 to 2015.The sequence analysis of RdRp regions showed that G Ⅱ.4 (37.50％,6/16) was the predominant epidemic genotype of norovirus during 2014,and G Ⅱ.17 (71.43％,20/28)became the predominant one during 2015.The 11 G Ⅱ.4 sequences of RdRp region obtained in this experiment and the G Ⅱ.4 reference strains which were isolated before 2009 were not in the same phylogenetic branch,but the G Ⅱ.4 experiment sequences and the reference strains which were isolated in recent years such as Sydney NSW0514 strain were in the same phylogenetic branch.The gene homologies of the G Ⅱ.4 experiment sequences and the older reference strains were more different than the experiment sequences and the younger reference strains.The 23 G Ⅱ.17 sequences of RdRp region obtained in this experiment were in the same phylogenetic branch with all the G [Ⅱ.17 reference strains selected.There were some nucleotide differences between the experiment sequences and the G Ⅱ.17 reference strains.All the experiment sequences have no amino acid differences with all the G Ⅱ.17 reference strains except 14115 and 15017.Conclusions The predominant epidemic genotype of norovirus was G Ⅱ.4 during 2014,which turned to G Ⅱ.17 during 2015 in adults with acute gastroenteritis in Shunyi District of Beijing.There were more than one norovirus genotype co-epidemic in Shunyi from 2014 to 2015.
