Construction and immune evaluation of the dendritic cell vaccine pulsed with HPV16L1
10.3760/cma.j.issn.1003-9279.2016.06.005
- VernacularTitle:负载HPV16L1抗原的DC疫苗的构建及免疫效果的研究
- Author:
Jing LUO
1
;
Ming YANG
;
Tiewei LIU
;
Tingjuan QI
;
Yi ZENG
;
Yubai ZHOU
Author Information
1. 100124,北京工业大学生命科学与生物工程学院病毒药理室
- Keywords:
Dendritic cell;
vaccine;
Papillomavirus human;
major capsid protein
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(6):516-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective Constructing the dendritic cell (DC) vaccine loading HPV16L1 antigen,and evaluating the specific humoral and cellular immune responses induced by DC-L1.Methods The mature dendritic cells (DCs) were induced in vitro from bone marrow precursors by co-culturing with cytokines cocktail,and then transduced with a recombinant adenovirus harboring the HPV16L1 gene to obtain the DC-L1 vaccine.C57BL/6 mouse were immunized intramuscularly by single dose of DC-L1 and recombinant adenovirus vaccine Adv-HPV16L1 respectively.The specific humoral and cellular immune responses were determined by quantitative ELISA and ELISPOT at different time points.Results DC-L1 cells exhibited typical morphological features of dendritic cells,and the expression of HPV16L1 can be detected by western blot.Both DC-L1 and Adv-HPV16L1 can induce specific cellular immune response,while with distinct kinetics.Single dose of Adv-HPV16L1 induced responses immediately,and attained the peak level at the third week after immunization,then diminished gradually.While responses induced by DC-L1 elevated slowly,and eventually exceeded that of recombinant adenovirus group at the fifth week of postimmunization.As to humoral immunity,both DC-L1 and Adv-HPV16L1 vaccines can induce specific humoral immune reponses,and with similar kinetics.Conclusion The DC-L1 vaccine was constructed successfully.The DC-L1 vaccine can induce high levels of HPV16L1 specific humoral and cellular immune response.
