Detection of EBV-LMP2-specific cell responses and EBV DNA copies in IgA/EBV-VCA positive individuals and patients with nasopharyngeal carcinoma
10.3760/cma.j.issn.1003-9279.2016.05.019
- VernacularTitle:IgA/EBV-VCA阳性人群和鼻咽癌患者的EB病毒潜伏膜蛋白2特异性细胞免疫及EBV DNA载量检测
- Author:
Zhenlian TAN
1
;
Yongli WANG
;
Zhiping MAI
;
Jian LIAO
;
Zhan WANG
;
Shaobing ZHAN
;
Haijun DU
Author Information
1. 643200,广西梧州市疾病预防控制中心
- Keywords:
IgA/ EBV-VCA;
latent membrane protein 2;
Cell responses;
EBV DNA
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(5):493-495
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between EBV-LMP2-specific cell responses,EBV DNA copies and titers of IgA antibody to viral capsid antigen (IgA/VCA).Methods IgA/VCA antibody were tested with immune-enzyme method.Both of plasma and lymphocytes were respectively separated from IgA/VCA-positive individuals and patients with nasopharyngeal carcinoma (NPC) without treatment.EBV DNA copies and LMP2-specific cell responses were detected by interferon-gamma Elispots assay and fluorescent quantitative PCR method.Results IgA/VCA antibody in 80 of 1 233 individuals was positive in Cangwu county,and the positive rate is 6％.EBV-LMP2-specific cell responses and EBV DNA copies were detected in 72 of 80 IgA/VCA positive individuals and patients with NPC.EBV DNA copies in plasma were increased with rise of titers of IgA/VCA antibody,while EBV-LMP2-specific cell responses were declined.Conclusion EBV-LMP2-specific cell responses and EBV DNA copies are probably associated with titers of IgA/VCA antibody.
