Adjuvants enhance cellular immune response induced by recombinant adenoviral vector encoding HIV-1 env gene in mice
10.3760/cma.j.issn.1003-9279.2016.05.011
- VernacularTitle:重组腺病毒载体HIV-1env疫苗合用佐剂的免疫效果研究
- Author:
Lian YU
1
;
Xiaozhou HE
;
Wandi WANG
;
Xia FENG
;
Ke XU
;
Yi ZENG
Author Information
1. 100124,北京工业大学生命科学与生物工程学院病毒与药理室
- Keywords:
HIV vaccine;
Adjuvant;
IL-7;
IL-21
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(5):461-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immune effects of IL-7,IL-21 as gene adjuvants,Poly(I:C) and CpG ODN for HIV vaccine.Methods Mouse interleukin 7 DNA adjuvant (pVR-IL7) and mouse interleukin 21 DNA adjuvant (pVR-IL21) were constructed.BALB/c mice received DNA prime-adenoviral vector boost immunization with pVR-HIVenv-Ad5-HIVenv alone or combined with pVR-IL7,pVR-IL21,Poly (I:C) and CpG ODN.Cellular and humoral immune responses were assessed by IFN-g enzyme-linked immunosorbent spot assay and enzyme-linked immunosorbent assay.Results Compared with those immunized with vaccines alone,the mice immunized with pVR-IL7 had increased specific cellular response (P ＜ 0.05),CpG ODN had synergic effects with IL7 as adjuvants (P ＜ 0.05) Conclusion IL7 but not IL21 can enhance the specific cellular immune response of Ad5-HIVenv in mice.
