Epidemiological characteristic and typing of human adenovirus in Wuxi area of 2011-2013
10.3760/cma.j.issn.1003-9279.2016.05.010
- VernacularTitle:2011-2013年无锡地区人腺病毒流行特征与分型
- Author:
Guangyuan MA
1
;
Ruixia LIU
;
Yong XIAO
;
Jing BAO
;
Yayong JI
;
Jialin WU
;
Xia LING
Author Information
1. 214023,无锡市疾病预防控制中心
- Keywords:
Human Adenovirus;
Epidemiological Characteristics;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(5):457-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the epidemiological characteristics and type of human adenovirus (HAdv) in Wuxi area in 2011-2013.Methods A total of 1983 acute respiratory infections samples were collected from Wuxi People's Hospital during 2011-2013.HAdv was detected by Real-time PCR,the positive samples were inoculated into Hep-2 cells to isolate the viruses.The amplificons were purified and sequenced,and the sequences were analyzed by Bioedit and MEGA 5.0.Results Human adenovirus was appeared throughout the year in Wuxi area during 2011-2013.In winter and spring,the highest rate (44.26％) was detected in 1-3 years old children.There were 4 strains (13.33％) for HAdV-3,6 strains for HAdV-7(20.00％) of B subgroup,5 strains (16.66％) for HAdV-1,8 strains (26.67％) for HAdV-2,3 strains (10.00％) for HAdV-5,4 strains (13.33％) for HAdV-6 of C subgroup.The phylogenetic tree formed two independent branches and C subgroup gradually became advantage.Conclusions Human adenovirus had obvious seasonal characteristics in Wuxi area during 2011-2013,mainly in winter and spring;1-3 years old children were the main susceptible population.Six types of B and C subgroups were the dominant epidemic strains,with the epidemic tendency transformed from subgroup B to C.
